• Product NamePHLDA2 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHuman,Rat
• SpecificityThe antibody detects endogenous level of total PHLDA2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PHLDA2.
• Target NamePHLDA2
• Alternative Names    IPL; BRW1C; BWR1C; HLDA2; TSSC3;
• Accession No. Swiss-Prot#: Q53GA4
  NCBI Gene ID: 7262
• UniprotQ53GA4
• Gene ID7262;
• SDS-PAGE MW 18 kD
• Concentration 1.0mg/ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000 
  IHC 1:100 - 1:500

This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver.

• Sample: A549 Cell Lysate at 30 ug; Primary: PHLDA2 antibody at 1/1000 dilution; Secondary: HRP Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 17 kD; Observed band size: 18 kD.
• Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PHLDA2 antibody at 1:200 overnight at 4°C;Secondary antibody HRP Goat Anti-Rabbit IgG was diluted at 1:200(room temperature, 30min), followed by DAB staining.
• Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PHLDA2 antibody at 1:200 overnight at 4°C; Secondary antibody HRP Goat Anti-Rabbit IgG was diluted at 1:200(room temperature, 30min), followed by DAB staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting