• Product NamePCBD1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PCBD1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PCBD1.
• Target NamePCBD1
• Alternative Names    PCD; PHS; DCOH; PCBD;
• Accession No. Swiss-Prot#: P61457
  NCBI Gene ID: 5092
• UniprotP61457
• Gene ID5092;
• SDS-PAGE MW 12kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:10 - 1:100

This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants.

• Immunofluorescence analysis of MCF7 cells using PCBD1 . Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using PCBD1 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting