• Product NameC21orf33 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total C21orf33 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human C21orf33.
• Target NameC21orf33
• Alternative Names    ES1; HES1; KNPH; KNPI; GT335;
• Accession No. Swiss-Prot#: P30042
  NCBI Gene ID: 8209
• UniprotA0A0B4J2D5
• Gene ID8209;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:10 - 1:100

This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants.

• Western blot analysis of extracts of various cell lines, using C21orf33 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
• Immunofluorescence analysis of U2OS cells using C21orf33 .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting