• Product NamePRAME antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of total PRAME protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PRAME.
• Target NamePRAME
• Alternative Names    MAPE; OIP4; CT130; OIP-4;
• Accession No. Swiss-Prot#: P78395
  NCBI Gene ID: 23532
• UniprotP78395
• Gene ID23532;
• SDS-PAGE MW 57kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:20 - 1:50

This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

• Immunofluorescence analysis of U2OS cells using PRAME . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting