• Product NameBLMH antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total BLMH protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human BLMH.
• Target NameBLMH
• Alternative Names    BH; BMH;
• Accession No. Swiss-Prot#: Q13867
  NCBI Gene ID: 642
• UniprotQ13867
• Gene ID642;
• SDS-PAGE MW 52kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:20 - 1:50

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.

• Immunofluorescence analysis of HeLa cells using BLMH . Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using BLMH at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting