• Product NameEEF1B2 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total EEF1B2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human EEF1B2.
• Target NameEEF1B2
• Alternative Names    EF1B; EEF1B; EEF1B1;
• Accession No. Swiss-Prot#: P24534
  NCBI Gene ID: 1933
• UniprotP24534
• Gene ID1933;
• SDS-PAGE MW 24kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

• Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of NIH/3T3 cells using EEF1B2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using EEF1B2 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting