• Product NameGLUD2 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total GLUD2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human GLUD2.
• Target NameGLUD2
• Alternative Names    GDH2; GLUDP1;
• Accession No. Swiss-Prot#: P49448
  NCBI Gene ID: 2747
• UniprotP49448
• Gene ID2747;
• SDS-PAGE MW 61kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:10 - 1:100

The protein encoded by this gene is localized to the mitochondrion and acts as a homohexamer to recycle glutamate during neurotransmission. The encoded enzyme catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate. This gene is intronless.

• Western blot analysis of extracts of various cell lines, using GLUD2 at 1:1000 dilution.
• Immunofluorescence analysis of A549 cells using GLUD2 . Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human liver cancer using GLUD2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human esophageal cancer using GLUD2 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using GLUD2 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human liver damage using GLUD2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human gastric cancer using GLUD2 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting