• Product NameLONP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total LONP1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human lon peptidase 1, mitochondrial
• Target NameLONP1
• Alternative Names    LON; LONP; PIM1; hLON; LonHS; PRSS15
• Accession No. Swiss-Prot:P36776
  Gene Accssion:NP_001263408
• UniprotP36776
• Gene ID9361;
• SDS-PAGE MW 106KD
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry:1:25-1:100

This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Hela, hepg2, NIH/3T3 and A431 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #40199 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #40199 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting