• Product NameNME2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total NME2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human NME/NM23 nucleoside diphosphate kinase 2
• Target NameNME2
• Alternative Names    PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
• Accession No. Swiss-Prot:P22392
  Gene Accssion:NP_002503
• UniprotP22392
• Gene ID4831;654364;
• SDS-PAGE MW 17KD
• Concentration 2.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry:1:30-1:150

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product.?

• Gel: 12%SDS-PAGE
  Lysates (from left to right): Hela, NIH/3T3, lncap and 293T cell, mouse brain tissue, A549 and Jurkat cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #40247 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting