• Product NameOTUD6A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total OTUD6A protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human OTUD6A
• Target NameOTUD6A
• Alternative Names    DUBA2; HSHIN6
• Accession No. Swiss-Prot#:Q7L8S5
  NCBI Gene ID:139562
  Gene Accssion:NP_997203
• UniprotQ7L8S5
• Gene ID139562;
• Calculated MW 33 kDa
• Concentration 1.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20C

• Western blotting:1:500-2000
  

Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA2 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane 1-4: Human breast cancer tissue, TM4, 231 and Jurkat cell lysates
  Primary antibody:OTUD6A Antibody at dilution 1/550
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting