• Product NameTPX2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TPX2 protein.
• Immunogen TypeProtein
• Immunogen DescriptionFusion protein of human TPX2, microtubule-associated
• Target NameTPX2
• Alternative Names    DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• Accession No. Swiss-Prot:Q9ULW0
  Gene Accssion:BC004136
• UniprotQ9ULW0
• Gene ID22974;
• SDS-PAGE MW 86KD
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry:1:30-1:150

Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): A431, 293T, hela and K562 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/800 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #40347 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting