• Product NameSPON1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total SPON1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human spondin 1, extracellular matrix protein
• Target NameSPON1
• Alternative Names    f-spondin; VSGP/F-spondin
• Accession No. Swiss-Prot:Q9HCB6
  Gene Accssion:NP_006099
• UniprotQ9HCB6
• Gene ID10418;
• SDS-PAGE MW 91KD
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry:1:25-1:100

F-Spondin, also designated Spondin-1 or vascular smooth muscle growth-promoting factor, is a member of the subgroup of the Thrombospondin type 1 class molecules. F-Spondin is a secreted, extracellular matrix-attached protein which patterns axonal trajectories by promoting adhesion and outgrowth of commissural axons, in addition to inhibiting outgrowth of motor axons.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Mouse brain and lung tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #40372 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting