• Product NameTPI1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide contain a sequence corresponding to a region within amino acids 187 and 249 of Human TPI1
• Target NameTPI1
• Accession No. Swiss-Prot:P60174
  Gene ID:7167
• UniprotP60174
• Gene ID7167;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 27kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  Immunofluorescence: 1:100-1:200
  

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

• Sample(30 ug of whole cell lysate)
  A: A431
  B: H1299
  12% SDS PAGE
  Primary antibody diluted at 1: 2000
• Immunohistochemical analysis of paraffin-embedded SNU-16 xenograft, using TPI1 antibody at 1: 100 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Triosephosphate isomerase antibody at 1: 500 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting