• Product NameeIF3K antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity purified by Protein A.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 133 (Q9UBQ5) of EIF3K
• Target NameeIF3K
• Accession No. Swiss-Prot:Q9UBQ5
  Gene ID:27335
• UniprotQ9UBQ5
• Gene ID27335;
• Concentration 10mg/ml
• Formulation Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 25kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

• Sample (30 ug of whole cell lysate)
  A: H1299
  12% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using eIF3K antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting