• Product NameEpoxide hydrolase 1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 193 of Epoxide hydrolase 1
• Target NameEpoxide hydrolase 1
• Accession No. Swiss-Prot:P07099
  Gene ID:2052
• UniprotP07099
• Gene ID2052;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 53kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:500
  Immunofluorescence: 1:100-1:200
  

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

• Sample (30 ug of whole cell lysate)
  A: Hela
  B: Hep G2
  10% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1 antibody at 1: 500 dilution.
• Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting