• Product NameBeclin1 (Phospho-Ser30) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsWB IHC IP
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Beclin1 only when phosphorylated at serine 30.
• Immunogen TypePeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 30 (D-T-S(p)-F-K) derived from Human Beclin1.
• Alternative Names    ATG6,VPS30,beclin1
• Accession No. Swiss-Prot#: Q14457
  NCBI Gene ID: 8678
  NCBI mRNA#: NM_003766.4
  NCBI Protein#: NP_003757.1
• UniprotQ14457
• Gene ID8678;
• Calculated MW 52KD
• Concentration 1.0mg/mL
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

•  WB dilution: 1:500~1:1000

   IHC dilution: 1:50~1:100

   IP dilution: 1:20~1:50

• Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration

Xu Qian1,et al. Phosphoglycerate kinase 1 phosphorylates Beclin1 to induce autophagy.Mol Cell. 2017 March 02; 65(5): 917–931.e6. doi:10.1016/j.molcel.2017.01.027. Autho

• The indicated His-PGK1 proteins immobilized on Ni-NTA agarose beads was incubated with Flag-ARD1 and Ac-CoA, followed by incubation with purified GST-Beclin1 or GST-Beclin1 S30A, in the presence of [γ- 32P] ATP. Autoradiography was performed.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting