PKC Polyclonal Antibody - SAB | Signalway Antibody

Catalog No.	Package Size	Price	Quantity
41347-1	50ul	$219.00
41347-2	100ul	$319.00

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Description

Product NamePKC Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC IF ELISA
Species ReactivityHu Ms Rt
SpecificityPKC Polyclonal Antibody detects endogenous levels of PKC protein.
Immunogen DescriptionSynthesized peptide derived from human PKC around the non-phosphorylation site of T497.
Target NamePKC
Alternative Names PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCB; PKCB; PRKCB1; Protein kinase C beta type; PKC-B; PKC-beta; PRKCD; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; PRKCE; PKCE; Protein kinase

Accession No. Swiss-Prot: P17252/P05771/Q05655/Q02156/P05129/P24723/Q04759/Q05513
NCBI Gene ID: 5578/5579/5580/5581/5582/5583/5588/5590
UniprotP17252
Gene ID5578;
SDS-PAGE MW 83kd
Concentration 1mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Store at -20˚C/1 year

Application Details

Images

Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody
Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,PKC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,PKC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PKC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,PKC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of human-liver tissue. 1,PKC Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-lung tissue. 1,PKC Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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PKC Polyclonal Antibody #41347

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