• Product NameTRIM28(phospho-Ser824) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
• ApplicationsWB, IHC
• Species ReactivityHu, Ms, Rt
• SpecificityThe antibody detects endogenous levels of total TRIM28(phospho-Ser824) protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide of human TRIM28(phospho-Ser824)
• Target NameTRIM28
• Alternative Names    KAP1; TF1B; RNF96; TIF1B; PPP1R157
• Accession No. Swiss-Prot#:Q13263
  NCBI Gene ID:10155
  Gene Accssion:NP_005753
• UniprotQ13263
• Gene ID10155;
• Calculated MW 110kD
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20˚C

• WB 1:500-2000;IHC 1:50-300

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008],

• Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim,using primary antibody at 1:1000 dilution.
• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting