• Product NameALCAM/CD166 Antibody
• Host SpeciesMouse
• Clone No.7C2
• PurificationProA affinity purified
• ApplicationsWB, IP, IF, IHC(P)
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionpeptide
• Alternative Names    Activated leukocyte cell adhesion molecule antibody
  ALCAM antibody
  ALCAM protein antibody
  CD 166 antibody
  CD166 antibody
  CD166 antigen antibody
  CD166_HUMAN antibody
  FLJ3851 antibody
  FLJ38514 antibody
  MEMD antibody
  MGC71733 antibody
  
• Accession No. Swiss-Prot#:Q13740
• UniprotQ13740
• Gene ID214;
• Calculated MW 100-105 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at 4˚C

• WB: 1:100-1:1,000
  IHC: 1:50-500
  
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

Activated leukocyte cell adhesion molecule (ALCAM), expressed on activated leukocytes, T cells, B cells and monocytes, is a member of the immunoglobulin superfamily (IgSF) and identified as a CD6 ligand. CD6 is a type I membrane protein in the scavenger receptor cysteine rich protein superfamily that acts in T cell adhesion and costimulation. ALCAM mediates thymocyte-thymic epithelial cell adhesion via homophilic (ALCAM-ALCAM) and heterophilic (ALCAMCD6) interactions involving a previously undescribed protein-protein interaction between a member of the scavenger receptor cysteine rich protein superfamily and the immunoglobulin superfamily.

• Western blot analysis of ALCAM expression in SK-N-SH whole cell lysate.
• Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing membrane staining of respiratory epithelial cells.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting