• Product NameLC3B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationImmunogen affinity purified
• ApplicationsWB,IF/ICC,IHC-p
• Species ReactivityHu, Ms, Rt
• SpecificityThe antibody detects endogenous MAP LC3β protein.
• Immunogen DescriptionRecombinant Protein of MAP LC3β
• Alternative Names    MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
• Accession No. Swiss-Prot#:Q9GZQ8
• UniprotQ9GZQ8
• Gene ID81631;
• Calculated MW 15 kDa
• Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• WB: 1:1000-2000
  IHC: 1:200-500
  IF: 1:200

microtubule associated protein 1 light chain 3 beta(MAP1LC3B) Homo sapiens The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],

• Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Rat Brain Tissue cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. （Red） GAPDH Monoclonal Antibody(2B8) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.
• Immunofluorescence analysis of Hela cell. 1,Cleaved-PARP-1 (D214) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). LC3B Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
• Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using MAP LC3β Polyclonal Antibody. Secondary antibody was diluted at 1:20000
• Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting