• Product NameEP-CAM Antibody
• Host SpeciesMouse
• Clone No.3-E1-E2
• PurificationProL affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu,Ms
• Immunogen Descriptionrecombinant protein
• Alternative Names    17 1A antibody
  323/A3 antibody
  Adenocarcinoma associated antigen antibody
  Adenocarcinoma-associated antigen antibody
  Antigen identified by monoclonal antibody
  AUA1 antibody
  AUA1 antibody
  CD326 antibody
  CD326 antigen antibody
  Cell surface glycoprotein Trop 1 antibody
  Cell surface glycoprotein Trop 2 antibody
  Cell surface glycoprotein Trop-1 antibody
  CO 17A antibody
  CO17 1A antibody
  CO17A antibody
  DIAR5 antibody
  EGP 2 antibody
  EGP antibody
  EGP2 antibody
  EGP314 antibody
  EGP40 antibody
  Ep CAM antibody
  Ep-CAM antibody
  EPCAM antibody
  EPCAM_HUMAN antibody
  EpCAM1 antibody
  Epithelial cell adhesion molecule antibody
  Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
  Epithelial cell surface antigen antibody
  Epithelial cellular adhesion molecule antibody
  Epithelial glycoprotein 1 antibody
  Epithelial glycoprotein 314 antibody
  Epithelial glycoprotein antibody
  ESA antibody
  GA733 1 antibody
  GA733 2 antibody
  GA733-2 antibody
  gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
  gp4 antibody
  hEGP 2 antibody
  hEGP314 antibody
  HNPCC8 antibody
  Human epithelial glycoprotein 2 antibody
  KS 1/4 antigen antibody
  KS1/4 antibody
  KSA antibody
  Ly74 antibody
  Lymphocyte antigen 74 antibody
  M1S 1 antibody
  M1S2 antibody
  M4S1 antibody
  Major gastrointestinal tumor associated protein GA733 2 antibody
  Major gastrointestinal tumor-associated protein GA733-2 antibody
  mEGP314 antibody
  Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
  Membrane component, chromosome 4, surface marker 1 antibody
  Membrane component, chromosome 4, surface marker antibody
  MIC18 antibody
  MK 1 antibody
  Protein 289A antibody
  TACD1 antibody
  TACSTD1 antibody
  TROP1 antibody
  Tumor associated calcium signal transducer 1 antibody
  Tumor associated calcium signal transducer 2 precursor antibody
  Tumor-associated calcium signal transducer 1 antibody
  
• Accession No. Swiss-Prot#:P16422
• UniprotP16422
• Gene ID4072;
• Calculated MW 35 kDa
• Concentration 2 mg/mL
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:200-1:500
  ICC: 1:100-1:200
  
  FC: 1:100-1:200

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. Mutations in EpCAM have also been associated with congenital tufting enteropathy which causes intractable diarrhea in newborn children. Specificity/Source: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM.

• Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution.
  
  
  Positive control:
  Lane1: Hela
  Lane2: NIH/3T3
  Lane3: MCF-7
  
  

• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained withhematoxylin.
• ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting