• Product NameCUB domain-containing protein 1 Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.B2-E6
• PurificationProtein affinity purified
• ApplicationsWB,IHC,ICC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein.
• Alternative Names    9030022E12Rik antibody
  AA409659 antibody
  CD 318 antibody
  CD_antigen=CD318 antibody
  CD318 antibody
  CD318 antigen antibody
  CDCP 1 antibody
  Cdcp1 antibody
  CDCP1_HUMAN antibody
  CUB domain containing protein 1 antibody
  CUB domain-containing protein 1 antibody
  E030027H19Rik antibody
  FLJ13772 antibody
  FLJ22375 antibody
  FLJ22969 antibody
  Membrane glycoprotein gp140 antibody
  MGC31813 antibody
  OTTHUMP00000164120 antibody
  OTTHUMP00000209568 antibody
  RGD1305578 antibody
  SIMA 135 antibody
  SIMA135 antibody
  Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
  Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
  Transmembrane and associated with src kinases antibody
  TRASK antibody
  
• Accession No. Swiss-Prot#:Q9H5V8
• UniprotQ9H5V8
• Gene ID64866;
• Calculated MW 92/72/37 kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  IHC: 1:50-1:100
  ICC: 1:50
  
  

CDCP1 (CUB-domain-containing protein 1) contains three extracellular CUB domains, a transmembrane domain, and two putative cytoplasmic tyrosine phosphorylation sites. Phosphorylation of the gp140 and p80 proteins is mediated by Src family kinases at various tyrosine residues, including Tyr 734. PTP family members mediate the desphosphorylation of CDCP1. The conversion of gp140 to p80 prolongs the phosphorylation state, which may affect signaling in epithelial wounds. CDCP1 acts as a marker for hematopoetic cells and also exhibits high expression in metastatic colon and breast tumors.

• Western blot analysis of CDCP1 on K562 cell lysates using anti-CDCP1 antibody at 1/200 dilution.
• Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
• ICC staining CDCP1 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CDCP1 (red) in A549 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting