• Product NameSMCP Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.D8-C10
• PurificationProA affinity purified
• ApplicationsWB, ICC
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein
• Alternative Names    HSMCSGEN1 antibody
  MCS antibody
  MCSP antibody
  MCSP_HUMAN antibody
  Mitochondrial capsule selenoprotein antibody
  SMCP antibody
  Sperm mitochondria associated cysteine rich protein antibody
  Sperm mitochondrial-associated cysteine-rich protein antibody
  
• Accession No. Swiss-Prot#:P49901
• UniprotP49901
• Gene ID4184;
• Calculated MW 13 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  
  ICC: 1:50-1:200
  
  

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The protein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath.

• Western blot analysis of SMCP on human SMCP recombinant protein using anti-SMCP antibody at 1/1,000 dilution.
• Western blot analysis of SMCP on HEK293 (1) and SMCP-hIgGFc transfected HEK293 (2) cell lysate using anti-SMCP antibody at 1/1,000 dilution.
• ICC staining SMCP (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting