• Product NameAMY1A Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.F6-G7
• PurificationProA affinity purified
• ApplicationsWB, FC
• Species ReactivityHu, Rt
• Immunogen DescriptionRecombinant protein
• Accession No. Swiss-Prot#:P04745
• UniprotP04745
• Gene ID276;277;278;
• Calculated MW 54 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  
  
  
  FC: 1:50-1:100

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.

• Western blot analysis of AMY1A on human AMY1A recombinant protein using anti-AMY1A antibody at 1/1,000 dilution.
• Western blot analysis of AMY1A on HEK293 (1) and AMY1A-hIgGFc transfected HEK293 (2) cell lysate using anti-AMY1A antibody at 1/1,000 dilution.
• Western blot analysis of AMY1A on A549 and C6 cell lysate using anti-AMY1A antibody at 1/1,000 dilution.
• Flow cytometric analysis of MCF-7 cells with AMY1A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting