• Product NameKLHL22 Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.A6-B10
• PurificationProA affinity purified
• ApplicationsWB,ICC
• Species ReactivityHu, Ms
• Immunogen DescriptionRecombinant protein
• Alternative Names    2610318I18Rik antibody
  kelch-like 22 antibody
  kelch-like family member 22 antibody
  Kelch-like protein 22 antibody
  KELCHL antibody
  KLH22_HUMAN antibody
  Klhl22 antibody
  RGD1306288 antibody
  
• Accession No. Swiss-Prot#:Q53GT1
• UniprotQ53GT1
• Gene ID84861;
• Calculated MW 72 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  
  ICC: 1:100-1:500
  
  

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.

• Western blot analysis of KLHL22 on mouse brain tissue lysate using anti-KLHL22 antibody at 1/1,000 dilution.
• ICC staining KLHL22 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting