• Product NameERGI3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPeptide affinity purified.
• ApplicationsWB,ICC,IHC,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic peptide within C-terminal human ERGI3.
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• Accession No. Swiss-Prot#:Q9Y282
• UniprotQ9Y282
• Gene ID51614;
• Calculated MW 55 kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Belongs to the ERGIC family. The ERGIC lies between the rough endoplasmic reticulum and Golgi on the secretory pathway. The vesicular-tubular cluster (VTC), also referred to as the ER-Golgi intermediate compartment (or ERGIC), is an organelle in eukaryotic cells. This compartment mediates trafficking between the endoplasmic reticulum and Golgi complex, facilitating the sorting of cargo. ERGI3 possible role in transport between endoplasmic reticulum and Golgi.

• Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution.
  Positive control��
  Lane1: SiHa
  Lane2: Human placenta
  Lane3: Rat bone marrow
  
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
• ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting