• Product NameRYR2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPeptide affinity purified.
• ApplicationsWB,ICC,IHC,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic peptide within human RYR2 aa 1450-1550.
• Alternative Names    Arrhythmogenic right ventricular dysplasia 2 antibody
  ARVC2 antibody
  ARVD2 antibody
  Brain ryanodine receptor calcium release channel antibody
  Brain type ryanodine receptor antibody
  Cardiac muscle ryanodine receptor calcium release channel antibody
  Cardiac muscle type ryanodine receptor antibody
  CCO antibody
  Central core disease of muscle antibody
  HBRR antibody
  hRYR 2 antibody
  hRYR2 antibody
  MHS antibody
  MHS1 antibody
  Ryanodine receptor 1 (skeletal) antibody
  Ryanodine receptor 1 antibody
  Ryanodine receptor 2 (cardiac) antibody
  Ryanodine receptor 2 antibody
  Ryanodine receptor 3 antibody
  Ryanodine receptor type1 antibody
  RYDR antibody
  RYR 1 antibody
  RYR 2 antibody
  RYR 3 antibody
  RYR antibody
  RYR-1 antibody
  RyR1 antibody
  RYR1_HUMAN antibody
  RYR2 antibody
  RYR3 antibody
  Sarcoplasmic reticulum calcium release channel antibody
  Skeletal muscle calcium release channel antibody
  Skeletal muscle ryanodine receptor antibody
  Skeletal muscle type ryanodine receptor antibody
  Skeletal muscle-type ryanodine receptor antibody
  SKRR antibody
  Type 1 like ryanodine receptor antibody
  Type 1 ryanodine receptor antibody
  VTSIP antibody
  
• Accession No. Swiss-Prot#:Q92736
• UniprotQ92736
• Gene ID6262;
• Calculated MW 565 kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  IHC: 1:50-1:200
  ICC: 1:50-1:100
  
  FC: 1:50-1:100

Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca2+ levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.

• Dot blot analysis of anti-RYR2 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR2 antibody was diluted with 1/500.
• Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR2 antibody. Counter stained with hematoxylin.
• ICC staining RYR2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining RYR2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining RYR2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting