• Product NameTGF-Beta 1 Antibody
• ClonalityMonoclonal
• PurificationProtein A affinity purified.
• ApplicationsWB, IHC-P, IF-Tissue
• Species ReactivityHuman, Mouse, Rat
• Immunogen DescriptionSynthetic peptide (KLH-coupled) corresponding to Human TGF-Beta 1
• Alternative Names    Cartilage-inducing factor antibody
  CED antibody
  Differentiation inhibiting factor antibody
  DPD1 antibody
  LAP antibody
  Latency-associated peptide antibody
  Prepro transforming growth factor beta 1 antibody
  TGF beta 1 antibody
  TGF beta antibody
  TGF beta 1 protein antibody
  TGF-beta 1 protein antibody
  TGF-beta-1 antibody
  TGF-beta-5 antibody
  TGF-beta1 antibody
  TGFB antibody
  Tgfb-1 antibody
  tgfb1 antibody
  TGFB1_HUMAN antibody
  TGFbeta antibody
  TGFbeta1 antibody
  Transforming Growth Factor b1 antibody
  Transforming Growth Factor beta 1 antibody
  Transforming growth factor beta 1a antibody
  transforming growth factor beta-1 antibody
  transforming growth factor, beta 1 antibody
  Transforming Growth Factor-?1 antibody
  
• Accession No. Swiss-Prot#:P01137
• UniprotP01137
• Gene ID7040;
• Calculated MW 44 kDa
• Concentration 1 mg/ml
• Formulation PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• WB 1:2,000
  IHC-P 1:200
  IF-Tissue 1:200

Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly.

• Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody at 1/2,000 dilution. Lane 1: Raji cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel.
• Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting