• Product NameCD3 epsilon Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPeptide affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms
• Immunogen DescriptionThis antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal residues of CD3 epsilon.
• Alternative Names    CD3 epsilon antibody
  CD3e antibody
  CD3e antigen epsilon polypeptide (TiT3 complex) antibody
  CD3E antigen epsilon polypeptide antibody
  CD3E antigen, epsilon subunit antibody
  CD3e molecule epsilon antibody
  CD3e molecule, epsilon (CD3 TCR complex) antibody
  CD3e molecule, epsilon (CD3-TCR complex) antibody
  CD3E_HUMAN antibody
  IMD18 antibody
  T cell antigen receptor complex epsilon subunit of T3 antibody
  T cell surface antigen T3/Leu 4 epsilon chain antibody
  T cell surface glycoprotein CD3 epsilon chain antibody
  T-cell surface antigen T3/Leu-4 epsilon chain antibody
  T-cell surface glycoprotein CD3 epsilon chain antibody
  T3E antibody
  TCRE antibody
  
• Accession No. Swiss-Prot#:P07766
• UniprotP07766
• Gene ID916;
• Calculated MW 23 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:200
  ICC: 1:200
  
  FC: 1:100-1:200

CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) These chains associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes.

• Western blot analysis of CD3E on different cell lysates using anti-CD3E antibody at 1/1000 dilution.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3E antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD3E antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD3E antibody. Counter stained with hematoxylin.
• ICC staining CD3E in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD3E in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Jurkat cells with CD3E antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting