• Product NameGAPDH Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.1-G2
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt, Monkey, Chicken, zebrafish
• Immunogen Descriptionrecombinant protein
• Alternative Names    38 kDa BFA-dependent ADP-ribosylation substrate antibody
  aging associated gene 9 protein antibody
  Aging-associated gene 9 protein antibody
  BARS-38 antibody
  cb609 antibody
  EC 1.2.1.12 antibody
  Epididymis secretory sperm binding protein Li 162eP antibody
  G3P_HUMAN antibody
  G3PD antibody
  G3PDH antibody
  GAPD antibody
  GAPDH antibody
  Glyceraldehyde 3 phosphate dehydrogenase antibody
  Glyceraldehyde-3-phosphate dehydrogenase antibody
  HEL-S-162eP antibody
  KNC-NDS6 antibody
  MGC102544 antibody
  MGC102546 antibody
  MGC103190 antibody
  MGC103191 antibody
  MGC105239 antibody
  MGC127711 antibody
  MGC88685 antibody
  OCAS, p38 component antibody
  OCT1 coactivator in S phase, 38-KD component antibody
  peptidyl cysteine S nitrosylase GAPDH antibody
  Peptidyl-cysteine S-nitrosylase GAPDH antibody
  wu:fb33a10 antibody
  
• Accession No. Swiss-Prot#:P04406
• UniprotP04406
• Gene ID2597;
• Calculated MW 36 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:10-1:100

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

• Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/1,000 dilution. Positive control:
  Lane 1: NCCIT
  Lane 2: PC12
  Lane 3: F9
• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
• ICC staining GAPDH in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining GAPDH in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting