• Product NameRho A+B+C Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SR38-00
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    AA017882 antibody
  AI324259, antibody
  Aplysia ras related homolog 9 (RhoC) antibody
  Aplysia ras-related homolog 12 antibody
  Aplysia RAS-related homolog 6 antibody
  Aplysia RAS-related homolog antibody
  ARH12 antibody
  ARH6 antibody
  ARH9 antibody
  ARHA antibody
  ARHB antibody
  ARHC antibody
  H12 antibody
  H6 antibody
  H9 antibody
  MGC117867 antibody
  MGC1448 antibody
  MGC61427 antibody
  MST081 antibody
  MSTP081 antibody
  Oncogene RHO H12 antibody
  Oncogene RHO H6 antibody
  Oncogene RHO H9 antibody
  OTTHUMP00000013675 antibody
  OTTHUMP00000013676 antibody
  OTTHUMP00000013802 antibody
  OTTHUMP00000013805 antibody
  OTTHUMP00000013807 antibody
  OTTHUMP00000013809 antibody
  OTTMUSP00000025086 antibody
  OTTMUSP00000025087 antibody
  Ras homolog 9 (RhoC) antibody
  Ras homolog gene family, member A antibody
  Ras homolog gene family, member B antibody
  Ras homolog gene family, member C antibody
  RAS-related homolog 9 antibody
  Rh -related GTP binding protein RhoC antibody
  Rho cDNA clone 12 antibody
  rho cDNA clone 6 antibody
  rho cDNA clone 9 antibody
  Rho related GTP binding protein RhoB antibody
  Rho related GTP binding protein RhoC antibody
  RHO12 antibody
  RHOA antibody
  RHOB antibody
  RHOC antibody
  rhoC GTPase antibody
  RHOH12 antibody
  RHOH6 antibody
  RHOH9 antibody
  RP11-426L16.4 antibody
  small GTP binding protein RhoA antibody
  Small GTP binding protein RhoC antibody
  Transforming protein RhoA antibody
  
• Accession No. Swiss-Prot#:P08134
• UniprotP08134
• Gene ID389;
• Calculated MW 24 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:200-1:500
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

• Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution. Positive control:
  Lane 1: HepG2
  Lane 2: Hela
  Lane 3: Jurkat
• Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
• ICC staining Rho A+B+C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Rho A+B+C in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Rho A+B+C in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of HepG2 cells with Rho A+B+C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting