• Product NameIKB alpha Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SZ00-07
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    I kappa B alpha antibody
  I-kappa-B-alpha antibody
  IkappaBalpha antibody
  IkB-alpha antibody
  IKBA antibody
  IKBA_HUMAN antibody
  IKBalpha antibody
  MAD 3 antibody
  MAD3 antibody
  Major histocompatibility complex enhancer-binding protein MAD3 antibody
  NF kappa B inhibitor alpha antibody
  NF-kappa-B inhibitor alpha antibody
  NFKBI antibody
  NFKBIA antibody
  Nuclear factor of kappa light chain gene enhancer in B cells antibody
  Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
  
• Accession No. Swiss-Prot#:P25963
• UniprotP25963
• Gene ID4792;
• Calculated MW 34 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

• Western blot analysis of IKB alpha on different lysates using anti-IKB alpha antibody at 1/1,000 dilution. Positive control:
  Lane 1: PC-12
  Lane 2: NIH/3T3
  Lane 3: Hela
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.
• ICC staining IKB alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining IKB alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining IKB alpha in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting