• Product NameCD20 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SY12-01
• PurificationProA affinity purified
• ApplicationsWB, IHC, IP
• Species ReactivityHu, Monkey
• Immunogen Descriptionrecombinant protein
• Alternative Names    APY antibody
  ATOPY antibody
  B lymphocyte antigen CD20 antibody
  B Lymphocyte Cell Surface Antigen B1 antibody
  B-lymphocyte antigen CD20 antibody
  B-lymphocyte cell-surface antigen B1 antibody
  B-lymphocyte surface antigen B1 antibody
  B1 antibody
  Bp 35 antibody
  Bp35 antibody
  CD 20 antibody
  CD20 antibody
  CD20 antigen antibody
  CD20 receptor antibody
  CD20_HUMAN antibody
  CVID5 antibody
  Fc epsilon receptor I beta chain antibody
  Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
  FCER1B antibody
  High affinity immunoglobulin epsilon receptor subunit beta antibody
  IgE Fc receptor subunit beta antibody
  IGEL antibody
  IGER antibody
  IGHER antibody
  LEU 16 antibody
  LEU16 antibody
  leukocyte surface antigen Leu 16 antibody
  Leukocyte surface antigen Leu-16 antibody
  Ly44 antibody
  Membrane spanning 4 domains A1 antibody
  Membrane spanning 4 domains subfamily A member 2 antibody
  Membrane-spanning 4-domains subfamily A member 1 antibody
  MS4A1 antibody
  MS4A2 antibody
  S7 antibody
  
• Accession No. Swiss-Prot#:P11836
• UniprotP11836
• Gene ID931;
• Calculated MW 33 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  
  
  

CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. The cytoplasmic domain of CD20 contains multiple phosphorylation sites, leading to additional isoforms. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. CD20 functions as a calcium-permeable cation channel, and it is known to accelerate the G0 to G1 progression induced by IGF-1. CD20 is activated by the IGF-1 receptor via the alpha subunits of the heterotrimeric G proteins. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.

• Western blot analysis of CD20 on Raji cell lysates using anti-CD20 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD20 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD20 antibody. Counter stained with hematoxylin.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting