• Product NamePhospho-Src(Y419) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.ST0800
• PurificationProA affinity purified
• ApplicationsWB, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic phospho-peptide corresponding to residues surrounding Tyr419 of human Src.
• Alternative Names    ASV antibody
  Avian sarcoma virus antibody
  c SRC antibody
  CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
  cSrc antibody
  EC 2.7.10.2 antibody
  Neuronal CSRC tyrosine specific protein kinase antibody
  Neuronal SRC antibody
  Oncogene SRC antibody
  OTTHUMP00000174476 antibody
  OTTHUMP00000174477 antibody
  p60 Src antibody
  p60-Src antibody
  p60Src antibody
  pp60c src antibody
  pp60c-src antibody
  pp60csrc antibody
  Proto oncogene tyrosine protein kinase Src antibody
  Proto-oncogene c-Src antibody
  Proto-oncogene tyrosine-protein kinase Src antibody
  Protooncogene SRC antibody
  Protooncogene SRC Rous sarcoma antibody
  Src antibody
  SRC Oncogene antibody
  SRC proto oncogene non receptor tyrosine kinase antibody
  SRC_HUMAN antibody
  SRC1 antibody
  Tyrosine kinase pp60c src antibody
  Tyrosine protein kinase SRC 1 antibody
  Tyrosine protein kinase SRC1 antibody
  v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
  V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
  v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
  
• Accession No. Swiss-Prot#:P12931
• UniprotP12931
• Gene ID6714;
• Calculated MW 60 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  
  
  
  FC: 1:50-1:100

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells. Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active.

• Flow cytometric analysis of Hela cells with Phospho-Src(Y419) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting