• Product NameATF5 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SD2099
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Activating transcription factor 5 alpha/beta antibody
  Activating transcription factor 5 antibody
  Activating transcription factor X antibody
  AFTA antibody
  ATF 5 antibody
  ATF 7 antibody
  ATF7 antibody
  ATFX antibody
  BZIP protein ATF7 antibody
  cAMP dependent transcription factor ATF 5 antibody
  cAMP dependent transcription factor ATF5 antibody
  Cyclic AMP dependent transcription factor ATF 5 antibody
  Cyclic AMP dependent transcription factor ATF5 antibody
  FLJ34666 antibody
  HMFN0395 antibody
  MGC102397 antibody
  NAP1 antibody
  NRIF3 associated protein antibody
  ODA 10 antibody
  Transcription factor ATFx antibody
  Transcription factor like protein ODA 10 antibody
  Transcription factor like protein ODA10 antibody
  
• Accession No. Swiss-Prot#:Q9Y2D1
• UniprotQ9Y2D1
• Gene ID22809;
• Calculated MW 31 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1.

• Western blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control:
  Lane 1: Jurkat
  Lane 2: PC-12
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
• ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting