• Product NameDysferlin Rabbit mAb
• Clone No.JF05-54
• PurificationAffinity-chromatography
• ApplicationsWB, ICC/IF, IHC
• Species ReactivityHu, Ms
• Immunogen DescriptionA synthesized peptide derived from human Dysferlin
• Alternative Names    DMAT antibody
  DYSF antibody
  DYSF_HUMAN antibody
  Dysferlin antibody
  Dysferlin limb girdle muscular dystrophy 2B (autosomal recessive) antibody
  Dysferlin limb girdle muscular dystrophy 2B antibody
  Dystrophy associated fer 1 like 1 antibody
  Dystrophy associated fer 1 like protein antibody
  Dystrophy associated fer1 like 1 antibody
  Dystrophy associated fer1 like protein antibody
  Dystrophy-associated fer-1-like protein antibody
  Fer 1 like protein 1 antibody
  Fer-1-like protein 1 antibody
  Fer1 like protein 1 antibody
  FER1L1 antibody
  FLJ00175 antibody
  FLJ90168 antibody
  LGMD 2B antibody
  LGMD2B antibody
  Limb girdle muscular dystrophy 2B (autosomal recessive) antibody
  Limb girdle muscular dystrophy 2B antibody
  Miyoshi myopathy antibody
  MM antibody
  MMD1 antibody
  
• Accession No. Swiss-Prot#:O75923
• UniprotO75923
• Gene ID8291;
• Calculated MW 237 kDa
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

• WB 1:1000-1:2000
  IHC 1:100-1:200
  ICC/IF 1:50-1:200

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.

• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
• ICC staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting