• Product NameHE4 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JF62-09
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms
• Immunogen Descriptionrecombinant protein
• Alternative Names    dJ461P17.6 antibody
  EDDM4 antibody
  epididymal protein 4 antibody
  Epididymal secretory protein E4 antibody
  Epididymis specific whey acidic protein type four disulfide core antibody
  HE 4 antibody
  Major epididymis specific protein E4 antibody
  Major epididymis-specific protein E4 antibody
  MGC57529 antibody
  Putative protease inhibitor WAP5 antibody
  WAP 5 antibody
  WAP domain containing protein HE4 antibody
  WAP domain containing protein HE4 V4 antibody
  WAP four disulfide core domain 2 antibody
  WAP four disulfide core domain protein 2 antibody
  WAP four-disulfide core domain protein 2 antibody
  WAP5 antibody
  WFDC 2 antibody
  WFDC2 antibody
  WFDC2_HUMAN antibody
  
• Accession No. Swiss-Prot#:Q14508
• UniprotQ14508
• Gene ID10406;
• Calculated MW 23 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

HE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them.

• Western blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control:
  Lane 1: SW480
  Lane 2: SKOV-3
• Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.
• ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining HE4 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SW480 cells with HE4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting