• Product NameATP5A1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM110-04
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    ATP synthase alpha chain, mitochondrial antibody
  ATP synthase subunit alpha antibody
  ATP synthase subunit alpha mitochondrial antibody
  ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
  ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
  ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
  ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
  ATP sythase (F1 ATPase) alpha subunit antibody
  ATP5A antibody
  Atp5a1 antibody
  ATP5AL2 antibody
  ATPA_HUMAN antibody
  ATPM antibody
  Epididymis secretory sperm binding protein Li 123m antibody
  hATP1 antibody
  HEL-S-123m antibody
  MC5DN4 antibody
  mitochondrial antibody
  Mitochondrial ATP synthetase antibody
  Mitochondrial ATP synthetase oligomycin resistant antibody
  Modifier of Min 2 mouse homolog antibody
  Modifier of Min 2, mouse, homolog of antibody
  MOM2 antibody
  OMR antibody
  ORM antibody
  OTTHUMP00000163475 antibody
  
• Accession No. Swiss-Prot#:P25705
• UniprotP25705
• Gene ID498;
• Calculated MW 50 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three α chains and three β chains, which are weakly homologous, as well as one γ chain, one δ chain and one e chain. F0 consists of three subunits: a, b and c. The α chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase α chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.

• Western blot analysis of ATP5A1 on different cells lysates using anti-ATP5A1 antibody at 1/500 dilution. Positive control��
  Line 1: Hela
  Line 2: MCF-7
• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
• ICC staining ATP5A1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ATP5A1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ATP5A1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with ATP5A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting