• Product NameRac1-2-3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM11-29
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Cell migration inducing gene 5 protein antibody
  EN 7 antibody
  OTTMUSP00000004488 antibody
  p21 Rac3 antibody
  p21-Rac3 antibody
  Rac1B antibody
  RAC3 antibody
  RAC3_HUMAN antibody
  RAS related C3 botulinum substrate 3 antibody
  Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody
  Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) antibody
  Ras-related C3 botulinum toxin substrate 3 antibody
  Rho family small GTP binding protein Rac3 antibody
  RP23-84C12.18 antibody
  EN-7 antibody
  EN7 antibody
  GX antibody
  HSPC 022 antibody
  HSPC022 antibody
  p21 Rac 2 antibody
  p21 Rac2 antibody
  p21-Rac2 antibody
  p21Rac2 antibody
  RAC 2 antibody
  Rac2 antibody
  RAC2_HUMAN antibody
  Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody
  Ras related C3 botulinum toxin substrate 2 antibody
  Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody
  Ras related C3 botulinum toxin substrate 3 antibody
  Ras-related C3 botulinum toxin substrate 2 antibody
  Rho family small GTP binding protein Rac 2 antibody
  Rho family small GTP binding protein Rac2 antibody
  Small G protein antibody
  Cell migration-inducing gene 5 protein antibody
  MGC111543 antibody
  MIG5 antibody
  Migration inducing gene 5 antibody
  Migration inducing protein 5 antibody
  p21 Rac1 antibody
  p21-Rac1 antibody
  Rac 1 antibody
  RAC1 antibody
  RAC1_HUMAN antibody
  Ras like protein TC25 antibody
  Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody
  Ras-like protein TC25 antibody
  Ras-related C3 botulinum toxin substrate 1 antibody
  Rho family small GTP binding protein Rac1 antibody
  TC 25 antibody
  TC25 antibody
  
• Accession No. Swiss-Prot#:P63000
• UniprotP63000
• Gene ID5879;
• Calculated MW 21/24 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

• Western blot analysis of RAC1/2/3 on MCF-7 cells lysates using anti-RAC1/2/3 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.
• ICC staining RAC1/2/3 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining RAC1/2/3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting