• Product NameCholine Acetyltransferase Rabbit mAb
• Clone No.JA67-11
• PurificationAffinity-chromatography
• ApplicationsWB, IP, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionA synthesized peptide derived from human CHAT
• Alternative Names    Acetyl CoA choline O acetyltransferase antibody
  Acetyl CoA:choline O acetyltransferase antibody
  ChAT antibody
  CHOACTase antibody
  Choline acetylase antibody
  choline acetyltransferase antibody
  Choline O acetyltransferase antibody
  Choline O-acetyltransferase antibody
  CLAT_HUMAN antibody
  CMS1A antibody
  CMS1A2 antibody
  EC 2.3.1.6 antibody
  OTTHUMP00000019583 antibody
  OTTHUMP00000019584 antibody
  
• Accession No. Swiss-Prot#:P28329
• UniprotP28329
• Gene ID1103;
• Calculated MW 82 kDa
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

• WB 1:1000-1:2000
  IHC 1:100-1:200
  ICC/IF 1:50-1:200
  IP 1:20-1:50
  FC 1:20-1:100

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.
• ICC staining Cho
  Line Acetyltransferase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Choline Acetyltransferase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
• Lane1 Mouse brain tissue lysate Use the Antibody at 1:2K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting