• Product NamePKC epsilon Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JA38-21
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    KPCE_HUMAN antibody
  MGC125656 antibody
  MGC125657 antibody
  nPKC epsilon antibody
  nPKC-epsilon antibody
  PKCE antibody
  Pkcea antibody
  PRKCE antibody
  Protein kinase C epsilon antibody
  Protein kinase C epsilon type antibody
  
• Accession No. Swiss-Prot#:Q02156
• UniprotQ02156
• Gene ID5581;
• Calculated MW 84 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

• Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1,000 dilution.
  Positive control��
  Lane1: Jurkat
  Lane2: MCF-7
  Lane3: HT-29
  
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
• ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY5Y cells with PKC epsilon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting