• Product NameSMYD3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    bA74P14.1 (novel protein) antibody
  bA74P14.1 antibody
  FLJ21080 antibody
  histone lysine N methyltransferase SMYD3 antibody
  KMT3E antibody
  MGC104324 antibody
  SET and MYND domain containing 3 antibody
  SET and MYND domain containing protein 3 antibody
  SET and MYND domain-containing protein 3 antibody
  SMYD 3 antibody
  Smyd3 antibody
  SMYD3 protein antibody
  SMYD3_HUMAN antibody
  Zinc finger MYND domain containing 1 antibody
  Zinc finger MYND domain containing protein 1 antibody
  Zinc finger MYND domain-containing protein 1 antibody
  Zinc finger protein subfamily 3A MYND domain containing 1 antibody
  Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
  ZMYND 1 antibody
  ZMYND1 antibody
  ZNFN3A1 antibody
  
• Accession No. Swiss-Prot#:Q9H7B4
• UniprotQ9H7B4
• Gene ID64754;
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  
  FC: 1:50-1:100

Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'.

• Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution. Positive control�� Lane1: Rat kidney
  Lane2: PC-12
  Lane3: Hela
  Lane4: Mouse spleen
  Lane5: NIH-3T3
  Lane6: Rat spleen
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
• ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting