• Product NameFructose 6 Phosphate Kinase Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JU53-31
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    6 Phosphofructokinase Muscle Type antibody
  6-phosphofructokinase antibody
  6-phosphofructokinase muscle type antibody
  EC 2.7.1.1 antibody
  EC 2.7.1.11 antibody
  GSD7 antibody
  K6PF_HUMAN antibody
  MGC8699 antibody
  muscle type antibody
  PFK, muscle type antibody
  PFK-A antibody
  PFKA antibody
  PFKL antibody
  PFKM antibody
  PFKP antibody
  PFKX antibody
  Phosphofructo 1 Kinase Isozyme A antibody
  Phosphofructo-1-kinase isozyme A antibody
  Phosphofructokinase 1 antibody
  Phosphofructokinase M antibody
  Phosphofructokinase, muscle antibody
  Phosphofructokinase, muscle type antibody
  Phosphofructokinase, polypeptide X antibody
  Phosphofructokinase-M antibody
  Phosphohexokinase antibody
  
• Accession No. Swiss-Prot#:P08237
• UniprotP08237
• Gene ID5213;
• Calculated MW 85 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.

• Western blot analysis of Fructose 6 Phosphate Kinase on PC-3M cell lysate using anti-Fructose 6 Phosphate Kinase antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
• ICC staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting