• Product NamePSME1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JU60-33
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    11S regulator complex alpha subunit antibody
  11S regulator complex subunit alpha antibody
  29 kD MCP activator subunit antibody
  29kD MCP activator subunit antibody
  Activator of multicatalytic protease subunit 1 antibody
  IFI5111 antibody
  IGUP I-5111 antibody
  Interferon gamma IEF SSP 5111 antibody
  Interferon gamma inducible protein 5111 antibody
  Interferon gamma up regulated I 5111 protein antibody
  Interferon gamma up-regulated I-5111 protein antibody
  MGC8628 antibody
  PA28a antibody
  PA28alpha antibody
  Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
  Proteasome activator 28 subunit alpha antibody
  Proteasome activator complex subunit 1 antibody
  Proteasome activator subunit 1 antibody
  PSME1 antibody
  PSME1_HUMAN antibody
  REG-alpha antibody
  REGalpha antibody
  
• Accession No. Swiss-Prot#:Q06323
• UniprotQ06323
• Gene ID5720;
• Calculated MW 27 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:100
  IP: 1:10-1:50
  

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

• Western blot analysis of PSME1 on MCF-7 cell (1) and rat lung tissue (2) lysate using anti-PSME1 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PSME1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PSME1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSME1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PSME1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PSME1 antibody. Counter stained with hematoxylin.
• ICC staining PSME1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PSME1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PSME1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting