• Product NameGRASP65 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JB36-34
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC,IP
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein
• Alternative Names    FLJ23443 antibody
  Golgi peripheral membrane protein p65 antibody
  Golgi phosphoprotein 5 antibody
  Golgi reassembly and stacking protein 1 antibody
  Golgi reassembly and stacking protein 65 kDa antibody
  Golgi reassembly and stacking protein, 65-kD antibody
  Golgi reassembly stacking protein 1 65kDa antibody
  Golgi reassembly stacking protein 1 antibody
  Golgi reassembly stacking protein of 65 kDa antibody
  Golgi reassembly-stacking protein 1 antibody
  Golgi reassembly-stacking protein of 65 kDa antibody
  GOLPH 5 antibody
  GOLPH5 antibody
  Gorasp 1 antibody
  GORASP1 antibody
  GORS1_HUMAN antibody
  GRASP 65 antibody
  GRASP65 antibody
  MGC118894 antibody
  MGC118897 antibody
  P65 antibody
  
• Accession No. Swiss-Prot#:Q9BQQ3
• UniprotQ9BQQ3
• Gene ID64689;
• Calculated MW 46 kDa (Predicted band size)
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes.

• Western blot analysis of GRASP65 on different cell lysates using anti-GRASP65 at 1/500 dilution.
  Positive control��
  Line 1: THP-1
  Line 2: SKBR3
  
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GRASP65 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human uterine tissue using anti-GRASP65 antibody. Counter stained with hematoxylin.
• ICC staining GRASP65 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining GRASP65 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining GRASP65 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of MCF-7 cells with GRASP65 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting