• Product NameNPHS2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JB51-33
• PurificationProA affinity purified
• ApplicationsWB,IHC,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
  nephrosis 2, idiopathic, steroid resistant antibody
  NPHS2 antibody
  NPHS2 gene antibody
  PDCN antibody
  PODO_HUMAN antibody
  Podocin antibody
  SRN1 antibody
  
• Accession No. Swiss-Prot#:Q9NP85
• UniprotQ9NP85
• Gene ID7827;
• Calculated MW 42 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  
  
  FC: 1:50-1:100

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

• Western blot analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
• Flow cytometric analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting