• Product NameGlucose 6 phosphate isomerase Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG33-86
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein corresponding to human Glucose 6 phosphate isomerase 300-500aa.
• Alternative Names    AMF antibody
  Aurocrine motility factor antibody
  Autocrine motility factor antibody
  DKFZp686C13233 antibody
  EC 5.3.1.9 antibody
  G6PI_HUMAN antibody
  Glucose phosphate isomerase antibody
  Glucose-6-phosphate isomerase antibody
  GNPI antibody
  GPI antibody
  Gpi1 antibody
  Hexose monophosphate isomerase antibody
  Hexosephosphate isomerase antibody
  Neuroleukin antibody
  NLK antibody
  Oxoisomerase antibody
  PGI antibody
  PHI antibody
  Phosphoglucose isomerase antibody
  Phosphohexomutase antibody
  Phosphohexose isomerase antibody
  Phosphosaccharomutase antibody
  SA 36 antibody
  SA-36 antibody
  SA36 antibody
  
• Accession No. Swiss-Prot#:P06744
• UniprotP06744
• Gene ID2821;
• Calculated MW 63 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment.

• Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
• ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting