• Product NameERp29 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG34-42
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within human ERp29 aa 1-150.
• Alternative Names    C12orf8 antibody
  Chromosome 12 open reading frame 8 antibody
  Endoplasmic reticulum lumenal protein ERp28 antibody
  Endoplasmic reticulum protein 29 antibody
  Endoplasmic reticulum protein 29 isoform 1 antibody
  Endoplasmic reticulum protein ERp29 antibody
  Endoplasmic reticulum resident protein 28 antibody
  Endoplasmic reticulum resident protein 29 antibody
  Endoplasmic reticulum resident protein 31 antibody
  Epididymis secretory protein Li 107 antibody
  ERp28 antibody
  ERP29_HUMAN antibody
  ERp31 antibody
  HEL S 107 antibody
  PDI-DB antibody
  PDIA9 antibody
  Protein disulfide isomerase family A member 9 antibody
  
• Accession No. Swiss-Prot#:P30040
• UniprotP30040
• Gene ID10961;
• Calculated MW 29 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

ERp29 is a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide-isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Western blot analysis of ERp29 on THP-1 cell using anti-ERp29 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp29 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-ERp29 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERp29 antibody. Counter stained with hematoxylin.
• ICC staining ERp29 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ERp29 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ERp29 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting