• Product NameNDUFB8 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG61-36
• PurificationProA affinity purified
• ApplicationsWB,IP,IHC,FC,ICC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within human NDUFB8 aa 30-130.
• Alternative Names    ASHI antibody
  CI-ASHI antibody
  Complex I ASHI subunit antibody
  Complex I-ASHI antibody
  mitochondrial antibody
  NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
  NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
  NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
  NADH-ubiquinone oxidoreductase ASHI subunit antibody
  NDUB8_HUMAN antibody
  NDUFB8 antibody
  
• Accession No. Swiss-Prot#:O95169
• UniprotO95169
• Gene ID4714;
• Calculated MW 17 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50
  IP: 1:10-1:50
  FC: 1:50-1:100

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

• Western blot analysis of NDUFB8 on different lysates using anti-NDUFB8 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: 293
  Lane 2: A549
  Lane 3: Mouse heart
  Lane 4: Rat spleen
  
• Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.
• Flow cytometric analysis of 293Tcells with NDUFB8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting