• Product NameUQCRFS1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG62-31
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC,IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within human UQCRFS1 aa 100 to the C-terminus.
• Alternative Names    Complex III subunit 5 antibody
  Complex III subunit IX antibody
  Cytochrome b c1 complex subunit Rieske mitochondrial antibody
  Cytochrome b-c1 complex subunit 11 antibody
  Cytochrome b-c1 complex subunit 5 antibody
  Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody
  petC antibody
  PGR1 antibody
  Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody
  Proton gradient regulation protein 1 antibody
  Rieske iron sulfur protein antibody
  Rieske iron-sulfur protein antibody
  RIP1 antibody
  RIS1 antibody
  RISP antibody
  Ubiquinol cytochrome C reductase rieske iron sulphur antibody
  Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody
  Ubiquinol-cytochrome c reductase 8 kDa protein antibody
  Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody
  UCRI_HUMAN antibody
  UQCR5 antibody
  UQCRFS1 antibody
  
• Accession No. Swiss-Prot#:P47985
• UniprotP47985
• Gene ID7386;
• Calculated MW 21 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:500-1:2,000
  
  FC: 1:10-1:50

Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Incorporation of UQCRFS1 is the penultimate step in complex III assembly.

• Western blot analysis of UQCRFS1 on different lysates using anti-UQCRFS1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: A549
  Lane 2: 293
  Lane 3: Mouse kidney
  
• Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.
• ICC staining UQCRFS1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining UQCRFS1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining UQCRFS1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting